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human gamma secretase  (Thermo Fisher)


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    Thermo Fisher human gamma secretase
    Human Gamma Secretase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+gamma+secretase/Apoferritin/pmc06250425-326-30-94
    Average 96 stars, based on 1 article reviews
    human gamma secretase - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: New tools for automated high-resolution cryo-EM structure determination in RELION-3
    Article Snippet: .. The following datasets were generated: Bai XC Scheres SHW Bai XC Yan C Yang G Lu P Ma D Sun L Zhou R Shi Y 2018 An atomic structure of human gamma-secretase [2925 multi-frame micrographs composed of 20 frames each in MRCS format] Electron Microscopy Public Image Archive EMPIAR-10194 Zivanov J Nakane T Hagen WJH Scheres SHW 2018 Human apo-ferritin reconstructed in RELION-3.0 Electron Microscopy Public Image Archive EMPIAR-10200 The following previously published datasets were used: Bartesaghi A Merk A Banerjee S Matthies D Wu X Milne JL Subramaniam S 2015 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor Electron Microscopy Public Image Archive EMPIAR-10061 Tan YZ Lyumkis D 2017 40 Degree Tilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer Electron Microscopy Public Image Archive EMPIAR-10097 Chen DH Afonine PV Jakana J Wang Z Haase-Pettingell C Jiang W Adams PD King JA Schmid MF Chiu W 2017 Bacteriophage P22 mature virion capsid protein Electron Microscopy Public Image Archive EMPIAR-10083 ..

    Electron Microscopy:

    Article Title: New tools for automated high-resolution cryo-EM structure determination in RELION-3
    Article Snippet: .. The following datasets were generated: Bai XC Scheres SHW Bai XC Yan C Yang G Lu P Ma D Sun L Zhou R Shi Y 2018 An atomic structure of human gamma-secretase [2925 multi-frame micrographs composed of 20 frames each in MRCS format] Electron Microscopy Public Image Archive EMPIAR-10194 Zivanov J Nakane T Hagen WJH Scheres SHW 2018 Human apo-ferritin reconstructed in RELION-3.0 Electron Microscopy Public Image Archive EMPIAR-10200 The following previously published datasets were used: Bartesaghi A Merk A Banerjee S Matthies D Wu X Milne JL Subramaniam S 2015 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor Electron Microscopy Public Image Archive EMPIAR-10061 Tan YZ Lyumkis D 2017 40 Degree Tilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer Electron Microscopy Public Image Archive EMPIAR-10097 Chen DH Afonine PV Jakana J Wang Z Haase-Pettingell C Jiang W Adams PD King JA Schmid MF Chiu W 2017 Bacteriophage P22 mature virion capsid protein Electron Microscopy Public Image Archive EMPIAR-10083 ..

    Single Particle:

    Article Title: New tools for automated high-resolution cryo-EM structure determination in RELION-3
    Article Snippet: .. The following datasets were generated: Bai XC Scheres SHW Bai XC Yan C Yang G Lu P Ma D Sun L Zhou R Shi Y 2018 An atomic structure of human gamma-secretase [2925 multi-frame micrographs composed of 20 frames each in MRCS format] Electron Microscopy Public Image Archive EMPIAR-10194 Zivanov J Nakane T Hagen WJH Scheres SHW 2018 Human apo-ferritin reconstructed in RELION-3.0 Electron Microscopy Public Image Archive EMPIAR-10200 The following previously published datasets were used: Bartesaghi A Merk A Banerjee S Matthies D Wu X Milne JL Subramaniam S 2015 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor Electron Microscopy Public Image Archive EMPIAR-10061 Tan YZ Lyumkis D 2017 40 Degree Tilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer Electron Microscopy Public Image Archive EMPIAR-10097 Chen DH Afonine PV Jakana J Wang Z Haase-Pettingell C Jiang W Adams PD King JA Schmid MF Chiu W 2017 Bacteriophage P22 mature virion capsid protein Electron Microscopy Public Image Archive EMPIAR-10083 ..



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    Fig. 2. Effect of PrPC on the expression of APP, BACE1, A40, sAPP, and sAPP. A) PrPC overexpressing HEK293 cells were analyzed 24 and 48 h after transfection by western blotting in comparison to control cells (1). Quantification of the band-intensity showed that PrPC was approximately 2-fold upregulated 24 h- and approximately 3.5 fold upregulated 48 h after transfection (1). Treatment with PNGase resulted in a deglycosylation of PrPC to prove the specificity of the PrPC detection (2). Concentration of PrPC was determined 48 h after transfection using a PrP <t>ELISA-assay</t> (3). B) Expression of APP and BACE1 was analyzed 24 and 48 h after transfection by western blotting. Both proteins were not influenced by the expression of PrPC. C) Amount of APP-cleavage fragments were measured via ELISA. Intra- and extracellular levels of A40, sAPP, and sAPP were decreased significantly in PrPC over-expressing cells (1–3). All in vitro experiments were carried out at least in triplicates. D) In mice, analysis of APP and BACE1 expression revealed no differences between Prnp0/0 and WT mice. E) ELISA measurement of A40 generation in mice brains revealed a marked, age-independent increase in Prnp0/0 mice compared to WT animals (1). PrPC overexpressing Tg35 mice revealed a decreased level of A40 (2) whereas concentration of <t>-secretase</t> remained unchanged in Prnp0/0 mice (3). All values were calculated in % of WT. Beta actin was used as an internal standard. For comparison between groups we used the student’s t-test (n = 5 per group in three independent experiments). Error bars indicate standard errors of the mean (SEM).
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    Fig. 2. Effect of PrPC on the expression of APP, BACE1, A40, sAPP, and sAPP. A) PrPC overexpressing HEK293 cells were analyzed 24 and 48 h after transfection by western blotting in comparison to control cells (1). Quantification of the band-intensity showed that PrPC was approximately 2-fold upregulated 24 h- and approximately 3.5 fold upregulated 48 h after transfection (1). Treatment with PNGase resulted in a deglycosylation of PrPC to prove the specificity of the PrPC detection (2). Concentration of PrPC was determined 48 h after transfection using a PrP ELISA-assay (3). B) Expression of APP and BACE1 was analyzed 24 and 48 h after transfection by western blotting. Both proteins were not influenced by the expression of PrPC. C) Amount of APP-cleavage fragments were measured via ELISA. Intra- and extracellular levels of A40, sAPP, and sAPP were decreased significantly in PrPC over-expressing cells (1–3). All in vitro experiments were carried out at least in triplicates. D) In mice, analysis of APP and BACE1 expression revealed no differences between Prnp0/0 and WT mice. E) ELISA measurement of A40 generation in mice brains revealed a marked, age-independent increase in Prnp0/0 mice compared to WT animals (1). PrPC overexpressing Tg35 mice revealed a decreased level of A40 (2) whereas concentration of -secretase remained unchanged in Prnp0/0 mice (3). All values were calculated in % of WT. Beta actin was used as an internal standard. For comparison between groups we used the student’s t-test (n = 5 per group in three independent experiments). Error bars indicate standard errors of the mean (SEM).

    Journal: Journal of Alzheimer's Disease

    Article Title: Impact of the Cellular Prion Protein on Amyloid-β and 3PO-Tau Processing

    doi: 10.3233/jad-130566

    Figure Lengend Snippet: Fig. 2. Effect of PrPC on the expression of APP, BACE1, A40, sAPP, and sAPP. A) PrPC overexpressing HEK293 cells were analyzed 24 and 48 h after transfection by western blotting in comparison to control cells (1). Quantification of the band-intensity showed that PrPC was approximately 2-fold upregulated 24 h- and approximately 3.5 fold upregulated 48 h after transfection (1). Treatment with PNGase resulted in a deglycosylation of PrPC to prove the specificity of the PrPC detection (2). Concentration of PrPC was determined 48 h after transfection using a PrP ELISA-assay (3). B) Expression of APP and BACE1 was analyzed 24 and 48 h after transfection by western blotting. Both proteins were not influenced by the expression of PrPC. C) Amount of APP-cleavage fragments were measured via ELISA. Intra- and extracellular levels of A40, sAPP, and sAPP were decreased significantly in PrPC over-expressing cells (1–3). All in vitro experiments were carried out at least in triplicates. D) In mice, analysis of APP and BACE1 expression revealed no differences between Prnp0/0 and WT mice. E) ELISA measurement of A40 generation in mice brains revealed a marked, age-independent increase in Prnp0/0 mice compared to WT animals (1). PrPC overexpressing Tg35 mice revealed a decreased level of A40 (2) whereas concentration of -secretase remained unchanged in Prnp0/0 mice (3). All values were calculated in % of WT. Beta actin was used as an internal standard. For comparison between groups we used the student’s t-test (n = 5 per group in three independent experiments). Error bars indicate standard errors of the mean (SEM).

    Article Snippet: We used a commercial Human -Secretase ELISA Kit (Cusabio, Wuhan, China) to analyze brain homogenates derived from Prnp0/0 and WT mice.

    Techniques: Expressing, Transfection, Western Blot, Comparison, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, In Vitro